Chromatography is a physical method of separation that distributes components to separate between two phases, one stationary (stationary phase), the other (the mobile phase) moving in a definite direction. The eluate is the mobile phase leaving the column. The eluent is the solvent that carries the analyte.
Also, how can chromatography be used to separate proteins?
Chromatography can be used to separate protein in solution or denaturing conditions by using porous gels. This technique is known as size exclusion chromatography. The principle is that smaller molecules have to traverse a larger volume in a porous matrix.
What is chromatography and how does it work?
Chromatography is used to separate mixtures of substances into their components. All forms of chromatography work on the same principle. They all have a stationary phase (a solid, or a liquid supported on a solid) and a mobile phase (a liquid or a gas).
What is chromatography and what is it used for?
Chromatography is a versatile separation technique widely used to obtain pure compounds from mixtures. All chromatographic techniques depend on a stationary phase, usually a finely divided solid or coated solid, that a mobile phase, usually a gas or liquid, moves through.